Oferta TFM | ID 36

Specific gene editing by CRISPR systems is a promising biotechnological tool for the treatment of different genetic diseases. However, the efficient and specific delivery of the system to the target tissues is still a key challenge in the clinical application of this technology. In this sense, our research is focused on the design of non-viral vehicles, based on lipid or metallic nanostructures, for the delivery of CRISPR proteins (Cas12, Cas9, Cas13) in complex with the guide RNA (RNP) to target disease related genes. The TFM student will be involved in the formation and characterization of the vehicles, and the evaluation of delivery activity and gene editing in in vitro assays using different cell lines. Throughout the project, the student will receive comprehensive training and gain experience in various laboratory techniques (recombinant protein expression and purification, nanoparticles formulation and characterization, cell biology, flow cytometry, qPCR, ddPCR, etc…). This project will provide practical experience in a highly relevant field of biotechnology, allowing the student to develop valuable skills for their future professional career.