Oferta TFM | ID 02

Both thyroid hormones (THs) and oxytocin (OXT) are two hormones well-known for their roles in regulating complex mood neurobehaviors such as fear, anxiety or depression. Interestingly, in the 90s it was discovered that TH stimulates OXT transcription, however, the relationship between THs and OXT has not been further explored.

Through a series of experiments in mice, this project will assess the effects of hypothyroidism on OXT production and explore its behavioural consequences. This project will:

1. Identify the molecular mechanisms by which THs regulate OXT production. This will be done by RNA sequencing analysis, quantitative PCR, RNAscope in situ hybridization, tissue immunolabelling and reporter gene assays.
2. Explore the behavioural consequences of TH-disrupted OXT production in different mouse models. This will include elevated plus maze, pup retrieval test and the tail suspension test among others.

In addition to the methods described above, the student will gain hands-on experience in the administration and monitoring of treatments in mice, tissue slicing using a cryostat, and RNA extraction.

This project provides an excellent opportunity to develop practical skills and contribute to research in the field of mood disorders and hormonal regulation.